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cellstar cell culture dishes  (Greiner Bio)


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    Structured Review

    Greiner Bio cellstar cell culture dishes
    Cellstar Cell Culture Dishes, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 95/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellstar+cell+culture+dish/Cell+Culture+Dish+Ps+145%2F20+Mm/bio_rxiv__2025__10__17__683000-116-6-14
    Average 95 stars, based on 182 article reviews
    cellstar cell culture dishes - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Suspension:

    Article Title: Neural crest related gene transcript regulation by valproic acid analogues in the cardiac embryonic stem cell test.
    Article Snippet: In vivo, neural crest (NC) cells contribute critically to heart formation.. The embryonic stem cells in the cardiac Embryonic Stem cell Test (ESTc) differentiate into a heterogeneous cell population including non-cardiomyocyte cells.. The use of molecular biomarkers from different mechanistic pathways can refine quantitative embryotoxicity assessment.

    Article Title: Molecular neural crest cell markers enable discrimination of organophosphates in the murine cardiac embryonic stem cell test
    Article Snippet: .. At differentiation day 0, hanging drops were formed by plating a 3.75·10 4 cells/mL suspension in 56 droplets of 20 μl to the lid of a 100/20 mm CELLSTAR® cell culture dish (Greiner Bio-One), which itself held 5 ml of ice-cold phosphate buffered saline (PBS; Ca 2+ , Mg 2+ free; Gibco). .. The hanging drops were kept warm at 37 0C and 5% CO 2 for 3 days and were relocated to a 60 mm bacterial petri dish (Greiner Bio-One) with 5 ml of exposure medium.

    Article Title: Cell differentiation in the cardiac embryonic stem cell test (ESTc) is influenced by the oxygen tension in its underlying embryonic stem cell culture.
    Article Snippet: Oxygen (O2) levels in the mammalian embryo range between 2.4% and 8%.. The cardiac embryonic stem cell test (ESTc) is a model for developmental toxicity predictions, which is usually performed under atmospheric O2 levels of 20%.. We investigated the chemical sensitivity of the ESTc carried out under 20% O2, using embryonic stem cells (ESC) cultured under either 20% O2 or 5% O2.

    Article Title: Genome-wide expression screening in the cardiac embryonic stem cell test shows additional differentiation routes that are regulated by morpholines and piperidines
    Article Snippet: .. For this method, a 3.75·10 4 cells/ml cell suspension was added in droplets to the inside of the lid of a 100/20 mm CELLSTAR® cell culture dish (Greiner Bio-One). .. 5 ml of ice-cold phosphate buffered saline (PBS; Ca 2+ , Mg 2+ free; Gibco) was added within the base of the culture dish and then lids were added after which the complete dishes were incubated for 3 days at 37 °C and 5 % CO 2 .

    Article Title: Gene regulation by morpholines and piperidines in the cardiac embryonic stem cell test.
    Article Snippet: The cardiac embryonic stem cell test (ESTc) is an in vitro embryotoxicity screen which uses cardiomyocyte formation as the main differentiation route.. Studies are ongoing into whether an improved specification of the biological domain can broaden the applicability of the test, e.g. to discriminate between structurally similar chemicals by measuring expression of dedicated gene transcript biomarkers.. We explored this with two chemical classes: morpholines (tridemorph; fenpropimorph) and piperidines (fenpropidin; spiroxamine).

    Cell Culture:

    Article Title: Neural crest related gene transcript regulation by valproic acid analogues in the cardiac embryonic stem cell test.
    Article Snippet: In vivo, neural crest (NC) cells contribute critically to heart formation.. The embryonic stem cells in the cardiac Embryonic Stem cell Test (ESTc) differentiate into a heterogeneous cell population including non-cardiomyocyte cells.. The use of molecular biomarkers from different mechanistic pathways can refine quantitative embryotoxicity assessment.

    Article Title: dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy.
    Article Snippet: Many disease-causing genes possess functionally equivalent counterparts, which are often expressed in distinct cell types.. An attractive gene therapy approach for inherited disorders caused by mutations in such genes is to transcriptionally activate the appropriate counterpart(s) to compensate for the missing gene function.. This approach offers key advantages over conventional gene therapies because it is mutationand gene size–independent.

    Article Title: Molecular neural crest cell markers enable discrimination of organophosphates in the murine cardiac embryonic stem cell test
    Article Snippet: .. At differentiation day 0, hanging drops were formed by plating a 3.75·10 4 cells/mL suspension in 56 droplets of 20 μl to the lid of a 100/20 mm CELLSTAR® cell culture dish (Greiner Bio-One), which itself held 5 ml of ice-cold phosphate buffered saline (PBS; Ca 2+ , Mg 2+ free; Gibco). .. The hanging drops were kept warm at 37 0C and 5% CO 2 for 3 days and were relocated to a 60 mm bacterial petri dish (Greiner Bio-One) with 5 ml of exposure medium.

    Article Title: Identifying essential long non-coding RNAs in cancer using CRISPRi-based dropout screens.
    Article Snippet: .. STAR Dobin et al.2 https://github.com/alexdobin/STAR NRSA Wang et al.3 https://zenodo.org/badge/latestdoi/680108742 CCTop Stemmer et al.4 https://bitbucket.org/juanlmateo/cctop_ standalone/src/master/ BLAT UC Santa Cruz https://users.soe.ucsc.edu/ kent/src/ Trimmomatic v.0.39 Bolger et al.5 http://www.usadellab.org/cms/?page=trimmomatic MAGeCK Li et al.6 https://bitbucket.org/liulab/mageck/src/master/ DESeq2 R package Love et al.7 https://bioconductor.org/packages/release/ bioc/html/DESeq2.html EnhancedVolcano R package Blighe et al.8 https://bioconductor.org/packages/release/ bioc/html/EnhancedVolcano.html Other Culture flask 50 mL 25 cm2 Greiner Bio-One Cat#690175 Culture flask 250 mL 75 cm2 Greiner Bio-One Cat#658175 CELLSTAR 6 well culture plates Greiner Bio-One Cat#657160 CELLSTAR Cell Culture Dish with Lid, 100 mm diameter Greiner Bio-One Cat#664160 CELLSTAR Cell Culture Dish with Lid, 145 mm diameter Greiner Bio-One Cat#639160 15 mL tubes Greiner Bio-One Cat#188271 50 mL tubes Greiner Bio-One Cat#227261 5 mL FACS tubes Corning Cat#352008 P1250 Long Reach Filter Tips Westburg Cat#WS5074 P200 Long Reach Filter Tips Westburg Cat#WS5040 P20 Long Reach Filter Tips Westburg Cat#WS5020 P10 Long Reach Filter Tips Westburg Cat#WS5010 1.5 mL tubes, DNAse/RNAse free Greiner Bio-One Cat#616201 Nonstick, RNase-free microfuge tubes, 1.5 mL Ambion, Thermo Fisher Scientific Cat#AM12450 ll OPEN ACCESS 4 STAR Protocols 4, 102588, December 15, 2023 Protocol ll OPEN ACCESSProtocol .. Equipment Source Identifier Panasonic CO2 incubator Panasonic Healthcare corporation MCO-170AICUVH-PE Hettich ROTINA 420R centrifuge Hettich zentrifugen Z723754 SH800 Cell Sorter Sony Biotechnology N/A Counting chamber N/A N/A Light microscope N/A N/A Thermomixer N/A N/A Stuart rotator SB3 Stuart N/A Bioanalyzer 2100 Agilent N/A Hardware Comment Benefit R32 GB of RAM To load the human genome in its entirety while mapping Improves performance R8 cores To use multithreading Improves performance drastically

    Article Title: Cell differentiation in the cardiac embryonic stem cell test (ESTc) is influenced by the oxygen tension in its underlying embryonic stem cell culture.
    Article Snippet: Oxygen (O2) levels in the mammalian embryo range between 2.4% and 8%.. The cardiac embryonic stem cell test (ESTc) is a model for developmental toxicity predictions, which is usually performed under atmospheric O2 levels of 20%.. We investigated the chemical sensitivity of the ESTc carried out under 20% O2, using embryonic stem cells (ESC) cultured under either 20% O2 or 5% O2.

    Article Title: Genome-wide expression screening in the cardiac embryonic stem cell test shows additional differentiation routes that are regulated by morpholines and piperidines
    Article Snippet: .. For this method, a 3.75·10 4 cells/ml cell suspension was added in droplets to the inside of the lid of a 100/20 mm CELLSTAR® cell culture dish (Greiner Bio-One). .. 5 ml of ice-cold phosphate buffered saline (PBS; Ca 2+ , Mg 2+ free; Gibco) was added within the base of the culture dish and then lids were added after which the complete dishes were incubated for 3 days at 37 °C and 5 % CO 2 .

    Article Title: Polytope: High-resolution epitope barcoding for in vivo spatial fate-mapping
    Article Snippet: Ears and tails from Rosa26 CreERT2/Polytope mice were sterilized in 70% ethanol for 30 seconds, washed in sterile PBS, cut into small pieces and placed into 15 mL tubes containing 10 mL sterile DMEM (without serum) supplemented with 0.5 mg/mL Liberase DL (Roche). .. Digestion was performed at 37°C for 90 minutes while shaking, followed by centrifugation at 300g, resuspension of the tissue pieces in 10 mL DMEM supplemented with 10% FSC and 1% penicillin-streptomycin–glutamine solution, and transfer to a 10 cm CELLSTAR cell culture dish (Greiner). ..

    Article Title: Gene regulation by morpholines and piperidines in the cardiac embryonic stem cell test.
    Article Snippet: The cardiac embryonic stem cell test (ESTc) is an in vitro embryotoxicity screen which uses cardiomyocyte formation as the main differentiation route.. Studies are ongoing into whether an improved specification of the biological domain can broaden the applicability of the test, e.g. to discriminate between structurally similar chemicals by measuring expression of dedicated gene transcript biomarkers.. We explored this with two chemical classes: morpholines (tridemorph; fenpropimorph) and piperidines (fenpropidin; spiroxamine).

    Chromatography:

    Article Title: dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy.
    Article Snippet: Many disease-causing genes possess functionally equivalent counterparts, which are often expressed in distinct cell types.. An attractive gene therapy approach for inherited disorders caused by mutations in such genes is to transcriptionally activate the appropriate counterpart(s) to compensate for the missing gene function.. This approach offers key advantages over conventional gene therapies because it is mutationand gene size–independent.

    Centrifugation:

    Article Title: dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy.
    Article Snippet: Many disease-causing genes possess functionally equivalent counterparts, which are often expressed in distinct cell types.. An attractive gene therapy approach for inherited disorders caused by mutations in such genes is to transcriptionally activate the appropriate counterpart(s) to compensate for the missing gene function.. This approach offers key advantages over conventional gene therapies because it is mutationand gene size–independent.

    Article Title: Polytope: High-resolution epitope barcoding for in vivo spatial fate-mapping
    Article Snippet: Ears and tails from Rosa26 CreERT2/Polytope mice were sterilized in 70% ethanol for 30 seconds, washed in sterile PBS, cut into small pieces and placed into 15 mL tubes containing 10 mL sterile DMEM (without serum) supplemented with 0.5 mg/mL Liberase DL (Roche). .. Digestion was performed at 37°C for 90 minutes while shaking, followed by centrifugation at 300g, resuspension of the tissue pieces in 10 mL DMEM supplemented with 10% FSC and 1% penicillin-streptomycin–glutamine solution, and transfer to a 10 cm CELLSTAR cell culture dish (Greiner). ..

    Imaging:

    Article Title: dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy.
    Article Snippet: Many disease-causing genes possess functionally equivalent counterparts, which are often expressed in distinct cell types.. An attractive gene therapy approach for inherited disorders caused by mutations in such genes is to transcriptionally activate the appropriate counterpart(s) to compensate for the missing gene function.. This approach offers key advantages over conventional gene therapies because it is mutationand gene size–independent.

    Microscopy:

    Article Title: dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy.
    Article Snippet: Many disease-causing genes possess functionally equivalent counterparts, which are often expressed in distinct cell types.. An attractive gene therapy approach for inherited disorders caused by mutations in such genes is to transcriptionally activate the appropriate counterpart(s) to compensate for the missing gene function.. This approach offers key advantages over conventional gene therapies because it is mutationand gene size–independent.

    Software:

    Article Title: dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy.
    Article Snippet: Many disease-causing genes possess functionally equivalent counterparts, which are often expressed in distinct cell types.. An attractive gene therapy approach for inherited disorders caused by mutations in such genes is to transcriptionally activate the appropriate counterpart(s) to compensate for the missing gene function.. This approach offers key advantages over conventional gene therapies because it is mutationand gene size–independent.

    Transferring:

    Article Title: dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy.
    Article Snippet: Many disease-causing genes possess functionally equivalent counterparts, which are often expressed in distinct cell types.. An attractive gene therapy approach for inherited disorders caused by mutations in such genes is to transcriptionally activate the appropriate counterpart(s) to compensate for the missing gene function.. This approach offers key advantages over conventional gene therapies because it is mutationand gene size–independent.

    Saline:

    Article Title: Molecular neural crest cell markers enable discrimination of organophosphates in the murine cardiac embryonic stem cell test
    Article Snippet: .. At differentiation day 0, hanging drops were formed by plating a 3.75·10 4 cells/mL suspension in 56 droplets of 20 μl to the lid of a 100/20 mm CELLSTAR® cell culture dish (Greiner Bio-One), which itself held 5 ml of ice-cold phosphate buffered saline (PBS; Ca 2+ , Mg 2+ free; Gibco). .. The hanging drops were kept warm at 37 0C and 5% CO 2 for 3 days and were relocated to a 60 mm bacterial petri dish (Greiner Bio-One) with 5 ml of exposure medium.

    FACS:

    Article Title: Identifying essential long non-coding RNAs in cancer using CRISPRi-based dropout screens.
    Article Snippet: .. STAR Dobin et al.2 https://github.com/alexdobin/STAR NRSA Wang et al.3 https://zenodo.org/badge/latestdoi/680108742 CCTop Stemmer et al.4 https://bitbucket.org/juanlmateo/cctop_ standalone/src/master/ BLAT UC Santa Cruz https://users.soe.ucsc.edu/ kent/src/ Trimmomatic v.0.39 Bolger et al.5 http://www.usadellab.org/cms/?page=trimmomatic MAGeCK Li et al.6 https://bitbucket.org/liulab/mageck/src/master/ DESeq2 R package Love et al.7 https://bioconductor.org/packages/release/ bioc/html/DESeq2.html EnhancedVolcano R package Blighe et al.8 https://bioconductor.org/packages/release/ bioc/html/EnhancedVolcano.html Other Culture flask 50 mL 25 cm2 Greiner Bio-One Cat#690175 Culture flask 250 mL 75 cm2 Greiner Bio-One Cat#658175 CELLSTAR 6 well culture plates Greiner Bio-One Cat#657160 CELLSTAR Cell Culture Dish with Lid, 100 mm diameter Greiner Bio-One Cat#664160 CELLSTAR Cell Culture Dish with Lid, 145 mm diameter Greiner Bio-One Cat#639160 15 mL tubes Greiner Bio-One Cat#188271 50 mL tubes Greiner Bio-One Cat#227261 5 mL FACS tubes Corning Cat#352008 P1250 Long Reach Filter Tips Westburg Cat#WS5074 P200 Long Reach Filter Tips Westburg Cat#WS5040 P20 Long Reach Filter Tips Westburg Cat#WS5020 P10 Long Reach Filter Tips Westburg Cat#WS5010 1.5 mL tubes, DNAse/RNAse free Greiner Bio-One Cat#616201 Nonstick, RNase-free microfuge tubes, 1.5 mL Ambion, Thermo Fisher Scientific Cat#AM12450 ll OPEN ACCESS 4 STAR Protocols 4, 102588, December 15, 2023 Protocol ll OPEN ACCESSProtocol .. Equipment Source Identifier Panasonic CO2 incubator Panasonic Healthcare corporation MCO-170AICUVH-PE Hettich ROTINA 420R centrifuge Hettich zentrifugen Z723754 SH800 Cell Sorter Sony Biotechnology N/A Counting chamber N/A N/A Light microscope N/A N/A Thermomixer N/A N/A Stuart rotator SB3 Stuart N/A Bioanalyzer 2100 Agilent N/A Hardware Comment Benefit R32 GB of RAM To load the human genome in its entirety while mapping Improves performance R8 cores To use multithreading Improves performance drastically



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    Reagents and tools table

    Journal: Molecular Systems Biology

    Article Title: Proteome-scale characterisation of motif-based interactome rewiring by disease mutations

    doi: 10.1038/s44320-024-00055-4

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: 145×20 cm Greiner Cellstar Cell Culture Dish , Greiner Bio-One , Cat. 639160.

    Techniques: Recombinant, Plasmid Preparation, Clone Assay, Produced, Sequencing, Magnetic Beads, Protease Inhibitor, Staining, Marker, Gel Extraction, Picogreen Assay, Affinity Chromatography, Purification, Transfection, Cell Culture, Software, Microscopy

    Reagents and tools table

    Journal: Molecular Systems Biology

    Article Title: Proteome-scale characterisation of motif-based interactome rewiring by disease mutations

    doi: 10.1038/s44320-024-00055-4

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: 145×20 cm Greiner Cellstar Cell Culture Dish , Greiner Bio-One , Cat. 639160.

    Techniques: Recombinant, Plasmid Preparation, Cloning, Produced, Sequencing, Magnetic Beads, Protease Inhibitor, Staining, Marker, Gel Extraction, Picogreen Assay, Affinity Chromatography, Purification, Transfection, Cell Culture, Software, Microscopy